Best Practices for Peptide Reconstitution

1. Always wear gloves when handling peptides to prevent contamination and degradation.
2. Allow peptide vials to reach room temperature before opening to prevent condensation that could affect peptide stability.
3. Pull the bacteriostatic water using the 3ml syringe housing and 18 gauge needle tip.
4. Add bacteriostatic water slowly down the side of the peptide vial rather than directly onto the peptide powder to prevent foaming and incomplete dissolution.
5. Gently swirl or rotate the vial to dissolve the peptide. Avoid vigorous shaking, which can cause foaming and potential degradation.

How to Transfer Rehydrated Peptides into an Injector Pen Cartridge (Safely)

Tools Needed:

• 1 rehydrated 3ml vial of peptide solution (BPC-157, TB500, etc)

• 1 sterile 3ml syringe (provided in Insulin Injector Pen + Setup)

• 1 sterile 20-gauge needle (yellow)

• 1 empty 3ml insulin injector pen cartridge

• Alcohol swabs

• Nitrile gloves (optional, but recommended for sterility)

Step-by-Step Instructions:

1. Sanitize Everything

   • Wipe down your work surface with alcohol.

   • Wash your hands thoroughly or wear gloves.

   • Wipe the rubber stopper of the vial and the fill port of the pen cartridge with an alcohol swab.

2. Draw the Peptide into the Syringe

   • Attach the 20-gauge needle to the 3ml syringe securely.

   • Insert the needle into the vial and slowly draw out the full amount of the rehydrated peptide solution (up to 3ml).

3. Prepare the Cartridge for Filling

   • Hold the 3ml insulin cartridge upright (tip up).

   • Ensure the cartridge is fully empty and the plunger is at the bottom.

4. Insert the Needle into the Cartridge Fill Port

   • Gently insert the needle into the cartridge’s fill port or rubber seal, ideally at a slight angle to avoid damaging the cartridge walls.

5. Begin Slow, Pulsed Transfer

   • Gently begin pushing a small amount of fluid into the cartridge (e.g., 0.3–0.5ml at a time).

   • DO NOT push hard or fast—this builds pressure and risks blowing out the back of the cartridge.

6. Pause and Equalize Pressure

   • After each push, stop and release pressure from the plunger.

   • This allows pressure from the cartridge to flow back into the syringe, equalizing the internal pressure (you will see bubbles going back into the syringe). 

7. Repeat the Pulse-Release Cycle

   • Continue this cycle:
     ➤ Push a small amount
     ➤ Stop and let pressure equalize
     ➤ Push again
     ➤ Repeat until the full peptide volume is transferred

   • Expect 5–7 small pulses to complete the full transfer.

8. Check for Air Bubbles

   • Once the cartridge is filled, gently tap it to move any air bubbles to the top, then express them if necessary (only if your cartridge design allows for it).

9. Remove the Needle Carefully

   • Once the transfer is complete, remove the needle slowly and recap it for safe disposal.

10. Label the Cartridge

• Immediately label the cartridge with the name of the peptide, dosage concentration, and date reconstituted.

11. Store Properly

• Store the filled cartridge in the refrigerator unless the product is shelf-stable.

⚠️ Important Warnings:

• Never force liquid into the cartridge—slow and steady wins here.

• If the plunger starts to slip or bulge outward, stop immediately and allow pressure to equalize.

• Always use sterile tools and clean surfaces to avoid contamination.

• Dispose of sharps responsibly in a puncture-resistant sharps container.

Common Peptide Reconstitution Mistakes

1. Incorrect calculations leading to inaccurate concentrations
2. Using inappropriate diluents for specific peptides
3. Vigorous shaking causing foam formation and peptide degradation
4. Neglecting temperature considerations during reconstitution
5. Repeated freeze-thaw cycles leading to degradation
6. Ignoring peptide-specific solubility information
7. Using contaminated diluents or non-sterile techniques